Distinct Subgroups of Cicer echinospermum Are Associated with Hybrid Sterility and Breakdown in Interspecific Crosses with Cultivated Chickpea

Crop wild relatives are a reservoir of phenotypic variation not present in the germplasm of cultivated species and thus have great potential for crop improvement. However, issues of genetic compatibility often interfere with effective utilization of crop wild relative taxa. Among chickpea (Cicer arietinum L.) crop wild relatives, Cicer echinospermum P.H. Davis is the sole species in the secondary genepool, being partially compatible with the primary genepool that is composed of the cultigen and its progenitor wild species Cicer reticulatum Ladizinksy. We report results from genetic studies among interspecific hybrids between cultivated chickpea and accessions from six recently identified wild C. echinospermum sites in southeastern Turkey, encompassing the known genetic diversity of the secondary genepool. Our studies indicate that both hybrid sterility and hybrid breakdown occur and are associated with distinct subgroups of C. echinospermum. Analysis of earlygeneration progenies suggests that both hybrid sterility and hybrid breakdown are conditioned by one to few genetic loci. These results clarify ambiguity in the nature of the hybridization barriers of reduced fertility in interspecific crossing of cultivated chickpea with C. echinospermum and should foster a more systematic and wider use of C. echinospermum for base broadening of cultivated chickpea. A. Kahraman, Dep. of Field Crops, Harran Univ., Sanliurfa, Turkey; A. Pandey and M.K. Khan, Dep. of Soil Science and Plant Nutrition, Selcuk Univ., Konya, Turkey; D. Lindsay and B. Tar’an, Dep. of Plant Science, Univ. of Saskatchewan, Saskatoon, SK; S. Moenga, L. Vance, E. Bergmann, N. Carrasquilla-Garcia, P.L. Chang, D.R. Cook, and R.V. Penmetsa, Dep. of Plant Pathology, Univ. of California at Davis, Davis, CA; M.-G. Shin and P.L. Chang, Dep. of Biological Sciences, Univ. of Southern California, Los Angeles, CA; E.J.B. von Wettberg, Dep. of Biology, Florida International Univ., Miami, FL; E.J.B. von Wettberg, Dep. of Plant and Soil Sciences, Univ. of Vermont, Burlington, VT; R.V. Penmetsa, Dep. of Plant Sciences, Univ. of California at Davis, Davis, CA. Received 2 June 2017. Accepted 16 Aug. 2017. *Corresponding authors ([email protected], [email protected]). Assigned to Associate Editor Dina St. Clair. Abbreviations: CWR, crop wild relative; QTL(s), quantitative trait loci. Published in Crop Sci. 57:3101–3111 (2017). doi: 10.2135/cropsci2017.06.0335 © Crop Science Society of America | 5585 Guilford Rd., Madison, WI 53711 USA This is an open access article distributed under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Published online October 13, 2017